SEPARATION OF SELECTED PESTICIDES BY AN HPLC TECHNIQUE; PERFORMANCE PARAMETERS AND VALIDATION

Abstract

The  aim  of  this  work  is  to  get  the  performance parameters  investigated  by  hight performance liquid chromatography (HPLC), for the separation method of 2,4-Dichlorophenoxiacetic acid,   3,6-Dichloro-2-methoxybenzoic   acid,   an   organic   mixture   with   herbicide   action.   The chromatographic  separation  with  better  peak  shape  was  achieved.  The  retention  times  (t R),  peak resolutions (R S ), separation factors ( Į ), column efficiency (N eff ), height of theoretical plates (HETP), indicate that the mobile phases in gradient, containing acetonitrile and water with 1%  acetic acid are the best for the  separation of investigated components on cromatographic column C18. Also it has been shown that in data conditions the methode is sensitive, precise and reproductible.